topflash and fopflash system Search Results


topflash and fopflash luciferase reporter  (Promega)
90
Promega topflash and fopflash luciferase reporter
Sfrp1 inhibits Wnt7a activity in the <t>TOPflash</t> luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 <t>and</t> <t>pcDNA3.1-Dkk1</t> , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.
Topflash And Fopflash Luciferase Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+and+fopflash+system/topflash+and+fopflash+luciferase+reporter/pmc06056652-69-14-19
Average 90 stars, based on 1 article reviews
topflash and fopflash luciferase reporter - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
topflash/fopflash firefly and sv-40 renilla luciferase constructs  (BioVector Inc)
90
BioVector Inc topflash/fopflash firefly and sv-40 renilla luciferase constructs
Sfrp1 inhibits Wnt7a activity in the <t>TOPflash</t> luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 <t>and</t> <t>pcDNA3.1-Dkk1</t> , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.
Topflash/Fopflash Firefly And Sv 40 Renilla Luciferase Constructs, supplied by BioVector Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+and+fopflash+system/topflash+fopflash+firefly+and+sv+40+renilla+luciferase+constructs/pm28272338-264-11-21
Average 90 stars, based on 1 article reviews
topflash/fopflash firefly and sv-40 renilla luciferase constructs - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
transfection plasmids topflash and fopflash  (Promega)
90
Promega transfection plasmids topflash and fopflash
Sfrp1 inhibits Wnt7a activity in the <t>TOPflash</t> luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 <t>and</t> <t>pcDNA3.1-Dkk1</t> , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.
Transfection Plasmids Topflash And Fopflash, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/topflash+and+fopflash+system/transfection+plasmids+topflash+and+fopflash/pmc07339185-126-18-24
Average 90 stars, based on 1 article reviews
transfection plasmids topflash and fopflash - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.

Journal: Frontiers in Molecular Neuroscience

Article Title: Opposite Roles of Wnt7a and Sfrp1 in Modulating Proper Development of Neural Progenitors in the Mouse Cerebral Cortex

doi: 10.3389/fnmol.2018.00247

Figure Lengend Snippet: Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.

Article Snippet: The Sfrp1 , Dkk1 coding sequences were subcloned into the pcDNA3.1 vector for the TOPflash and FOPflash luciferase reporter (Promega, United States) assay.

Techniques: Activity Assay, Luciferase, Reporter Assay, Activation Assay, Binding Assay, Expressing, Control, Plasmid Preparation, Mutagenesis, Transfection, Comparison